LncRNA ANRIL negatively regulated chitooligosaccharide-induced radiosensitivity in colon cancer cells by sponging miR-181a-5p

Background The radiosensitivity of colon cancer cells can be regulated by noncoding RNAs. Objectives In this study, the lncRNA antisense non-coding RNA in INK4 locus (ANRIL) was selected to analyze its regulatory role chitooligosaccharides (COS)-related cells. Material and methods ANRIL expression cell lines examined using real-time quantitative polymerase chain reaction (RT-qPCR), based on which we line that presented highest for research. were exposed X-rays (0 Gy, 2 4 6 Gy) evaluated changes miR-181a-5p RT-qPCR. Cell viability CCK8 method, while apoptosis detected with flow cytometry assays. Dual luciferase assays validated binding between miR-181a-5p. survival rates after differential COS treatments mg/mL, 1.0 2.0 3.0 4.0 5.0 mg/mL) rated assay. strongest dosage (5.0 further investigate ANRIL/miR-181a-5p modulating observed CCK-8 Results highly expressed lines, especially SW480 line. Irradiation significantly decreased a dose-dependent manner. overexpression reduced rate irradiation. MiR-181a-5p directly bound upregulated irradiation suppression apoptosis. treatment notably downregulated promoted partially reversed COS-induced inhibition rate; upregulation could counteract impact regulation Conclusions negatively induced sponging